By F. J. Baker
Finished undergraduate textbook at the rules and perform of labor in scientific laboratories. encompasses a common advent, and chapters on: scientific chemistry,cellular pathology, microbiology, hematology, and transfusion technological know-how. Halftone illustrations. prior version: c1985. Softcover. DNLM: Laboratory concepts and tactics.
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The angle ABY is therefore termed the 45 ELEMENTARY MICROSCOPY angle of incidence, and CBX the angle of refraction. The sine of the angle of incidence divided by the sine of the angle of refraction is a constant quantity for any two given media and is called the Refractive Index. In Fig. 34a the sine of the angle ABY divided by the sine of angle CBX determines the refractive index of the glass. (« («) FIG. 34. Diagrams to illustrate refraction of light rays passing through (a) a glass plate, and (b) a bi-convex lens.
38 CUTTING OF GLASS TUBING Rubber tubing, closed at one end, is useful for temporarily sealing the free end of the glass tube under manipulation. CUTTING OF GLASS TUBING When cutting tubing of 10 millimetres or less in diameter, the knife or file should be drawn across the glass at right angles, in a firm even movement, to make a short etched line. It is not necessary to saw the glass, as this tends to blunt the knife, and often results in a jagged edge. To break the tubing at the etched mark, it is held in both hands and bent while at the same time pulled in one motion (Fig.
The body tube is permanently fixed to the limb, and focusing is performed by racking the stage up or down. 53 ELEMENTARY MICROSCOPY MAGNIFICATION At the normal optical tube length of 160 millimetres, the total magnifying power of the compound microscope is the product of the magnification of the objective and eye-piece ; for example, with objective x 40 and eye-piece x 10, the magnification would be 40 x 10 = 400. If the tube length is varied, the final magnification is /Magnification of\ \ objective / /Magnification of \ \ eye-piece ) / Working tube length \ \Normal optical tube length/ For example, with objective X 40, eye-piece x 10 and working tube length 180 millimetres, total magnification is 180 40 X 10 X r ^ = 450 loi) If objectives are marked with focal length only, total magnification is /Magnification of\ \ eye-piece / / Working tube length \ \Focal length of objective/ For example, with eye-piece x 10, objective of focal length 4 millimetres and working tube length of 160 millimetres, total magnification is 10 x 160 — ^ — =400 SETTING UP THE MICROSCOPE It is of the utmost importance that the microscope should be correctly set up in order to obtain the best possible results.
An Introduction to Medical Laboratory Technology by F. J. Baker