By Morris J., Lycett J.
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Additional resources for A Monograph of the Mollusca from the Great Oolite, Chiefly from Minchinhampton and the Coast of Yorkshire, Part I: Univalves
3–10 g AT is obtained. With panniculectomy, a large chunk of AT, often with skin attached, is obtained and thus, it is necessary to dissect AT using scissors and forceps. Note: AT can be stored at 4 C overnight and ASCs can be isolated the next day. The yields, however, may be lower. 2. Mince AT into small pieces, approximately 5–10 mg pieces (1–2 mm3) using sharp scissors. Tissues from fat aspirations are already fragmented and do not require mincing. 3. Pour the minced tissue through a 250 mm mesh.
Optional) Stain nuclei with Weigert’s hematoxylin. Wash with slides for 10 min in running tap water. 3. Stain in solution A for 1 h. 4. Wash in two changes of solution B. 5. Physically remove most of the water from the slides. 6. Dehydrate in three changes of 100% ethanol. 7. Clear in xylene and mount in a resinous medium. 2 Method 1. Culture preadipocytes (3T3-L1 or primary vascular stromal cells) on a Lab-Tek Chamber. 2. Fix cells in 4% paraformaldehyde for 20 min, and quench excess paraformaldehyde with 100 mM glycine for 15 min and wash with PBS.
Et al. (2009). Induced pluripotent stem cells from a spinal muscular atrophy patient. Nature, 457, 277–280. Hotamisligil, G. S. (2006). Inflammation and metabolic disorders. Nature, 444, 860–867. , Frangioni, J. , Gygi, S. , et al. (2009). Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex. Nature, 460, 1154–1158. Miner, J. L. (2004). The adipocyte as an endocrine cell. Journal of Animal Science, 82, 935–941. , Parker, J. , Lugus, J. , & Walsh, K. (2011).
A Monograph of the Mollusca from the Great Oolite, Chiefly from Minchinhampton and the Coast of Yorkshire, Part I: Univalves by Morris J., Lycett J.